pegfp n1 Search Results


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Addgene inc human eb1 plasmid
Figure 6. Neo1 knockdown results in longer and faster comets of the microtubule plus end binding protein <t>EB1.</t> (a) Immunostaining of Caco-2 cells for EB1 (green) and alpha-tub (red). (b) Morphometric analysis of EB1 comets showed significantly higher aspect ratio (i.e. longer EB1 comets in Neo1-knockdown cells (1.97) compared to controls (1.93), p-value < 0.0001. (c) Western blots of EB1 shows no significant change in total protein levels in control and Neo1-siRNA treated cells. Full length blots for EB1 are represented in Supplementary Fig. S7. (d) EB1 comets speed analysis using Imaris showed faster EB1 comets (0.13 μm/sec) after Neo1 knockdown as compared to control cells (0.10 μm/sec), p-value = 0.0001. (e) EB1 comets track length and duration was significantly longer in Neo1-siRNA treated cells, p-value < 0.0001.
Human Eb1 Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Figure 6. Neo1 knockdown results in longer and faster comets of the microtubule plus end binding protein <t>EB1.</t> (a) Immunostaining of Caco-2 cells for EB1 (green) and alpha-tub (red). (b) Morphometric analysis of EB1 comets showed significantly higher aspect ratio (i.e. longer EB1 comets in Neo1-knockdown cells (1.97) compared to controls (1.93), p-value < 0.0001. (c) Western blots of EB1 shows no significant change in total protein levels in control and Neo1-siRNA treated cells. Full length blots for EB1 are represented in Supplementary Fig. S7. (d) EB1 comets speed analysis using Imaris showed faster EB1 comets (0.13 μm/sec) after Neo1 knockdown as compared to control cells (0.10 μm/sec), p-value = 0.0001. (e) EB1 comets track length and duration was significantly longer in Neo1-siRNA treated cells, p-value < 0.0001.
Pegfp, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Figure 6. Neo1 knockdown results in longer and faster comets of the microtubule plus end binding protein <t>EB1.</t> (a) Immunostaining of Caco-2 cells for EB1 (green) and alpha-tub (red). (b) Morphometric analysis of EB1 comets showed significantly higher aspect ratio (i.e. longer EB1 comets in Neo1-knockdown cells (1.97) compared to controls (1.93), p-value < 0.0001. (c) Western blots of EB1 shows no significant change in total protein levels in control and Neo1-siRNA treated cells. Full length blots for EB1 are represented in Supplementary Fig. S7. (d) EB1 comets speed analysis using Imaris showed faster EB1 comets (0.13 μm/sec) after Neo1 knockdown as compared to control cells (0.10 μm/sec), p-value = 0.0001. (e) EB1 comets track length and duration was significantly longer in Neo1-siRNA treated cells, p-value < 0.0001.
Pegfp N1, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Addgene inc plasmid
Figure 6. Neo1 knockdown results in longer and faster comets of the microtubule plus end binding protein <t>EB1.</t> (a) Immunostaining of Caco-2 cells for EB1 (green) and alpha-tub (red). (b) Morphometric analysis of EB1 comets showed significantly higher aspect ratio (i.e. longer EB1 comets in Neo1-knockdown cells (1.97) compared to controls (1.93), p-value < 0.0001. (c) Western blots of EB1 shows no significant change in total protein levels in control and Neo1-siRNA treated cells. Full length blots for EB1 are represented in Supplementary Fig. S7. (d) EB1 comets speed analysis using Imaris showed faster EB1 comets (0.13 μm/sec) after Neo1 knockdown as compared to control cells (0.10 μm/sec), p-value = 0.0001. (e) EB1 comets track length and duration was significantly longer in Neo1-siRNA treated cells, p-value < 0.0001.
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Figure 6. Neo1 knockdown results in longer and faster comets of the microtubule plus end binding protein <t>EB1.</t> (a) Immunostaining of Caco-2 cells for EB1 (green) and alpha-tub (red). (b) Morphometric analysis of EB1 comets showed significantly higher aspect ratio (i.e. longer EB1 comets in Neo1-knockdown cells (1.97) compared to controls (1.93), p-value < 0.0001. (c) Western blots of EB1 shows no significant change in total protein levels in control and Neo1-siRNA treated cells. Full length blots for EB1 are represented in Supplementary Fig. S7. (d) EB1 comets speed analysis using Imaris showed faster EB1 comets (0.13 μm/sec) after Neo1 knockdown as compared to control cells (0.10 μm/sec), p-value = 0.0001. (e) EB1 comets track length and duration was significantly longer in Neo1-siRNA treated cells, p-value < 0.0001.
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Figure 6. Neo1 knockdown results in longer and faster comets of the microtubule plus end binding protein <t>EB1.</t> (a) Immunostaining of Caco-2 cells for EB1 (green) and alpha-tub (red). (b) Morphometric analysis of EB1 comets showed significantly higher aspect ratio (i.e. longer EB1 comets in Neo1-knockdown cells (1.97) compared to controls (1.93), p-value < 0.0001. (c) Western blots of EB1 shows no significant change in total protein levels in control and Neo1-siRNA treated cells. Full length blots for EB1 are represented in Supplementary Fig. S7. (d) EB1 comets speed analysis using Imaris showed faster EB1 comets (0.13 μm/sec) after Neo1 knockdown as compared to control cells (0.10 μm/sec), p-value = 0.0001. (e) EB1 comets track length and duration was significantly longer in Neo1-siRNA treated cells, p-value < 0.0001.
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Figure 6. Neo1 knockdown results in longer and faster comets of the microtubule plus end binding protein <t>EB1.</t> (a) Immunostaining of Caco-2 cells for EB1 (green) and alpha-tub (red). (b) Morphometric analysis of EB1 comets showed significantly higher aspect ratio (i.e. longer EB1 comets in Neo1-knockdown cells (1.97) compared to controls (1.93), p-value < 0.0001. (c) Western blots of EB1 shows no significant change in total protein levels in control and Neo1-siRNA treated cells. Full length blots for EB1 are represented in Supplementary Fig. S7. (d) EB1 comets speed analysis using Imaris showed faster EB1 comets (0.13 μm/sec) after Neo1 knockdown as compared to control cells (0.10 μm/sec), p-value = 0.0001. (e) EB1 comets track length and duration was significantly longer in Neo1-siRNA treated cells, p-value < 0.0001.
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Reagents used in the investigation.
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Addgene inc codon optimized human lyn encoding plasmid pegfp n1 human lyn gfp
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Image Search Results


Figure 6. Neo1 knockdown results in longer and faster comets of the microtubule plus end binding protein EB1. (a) Immunostaining of Caco-2 cells for EB1 (green) and alpha-tub (red). (b) Morphometric analysis of EB1 comets showed significantly higher aspect ratio (i.e. longer EB1 comets in Neo1-knockdown cells (1.97) compared to controls (1.93), p-value < 0.0001. (c) Western blots of EB1 shows no significant change in total protein levels in control and Neo1-siRNA treated cells. Full length blots for EB1 are represented in Supplementary Fig. S7. (d) EB1 comets speed analysis using Imaris showed faster EB1 comets (0.13 μm/sec) after Neo1 knockdown as compared to control cells (0.10 μm/sec), p-value = 0.0001. (e) EB1 comets track length and duration was significantly longer in Neo1-siRNA treated cells, p-value < 0.0001.

Journal: Scientific reports

Article Title: Loss of Neogenin1 in human colorectal carcinoma cells causes a partial EMT and wound-healing response.

doi: 10.1038/s41598-019-40886-y

Figure Lengend Snippet: Figure 6. Neo1 knockdown results in longer and faster comets of the microtubule plus end binding protein EB1. (a) Immunostaining of Caco-2 cells for EB1 (green) and alpha-tub (red). (b) Morphometric analysis of EB1 comets showed significantly higher aspect ratio (i.e. longer EB1 comets in Neo1-knockdown cells (1.97) compared to controls (1.93), p-value < 0.0001. (c) Western blots of EB1 shows no significant change in total protein levels in control and Neo1-siRNA treated cells. Full length blots for EB1 are represented in Supplementary Fig. S7. (d) EB1 comets speed analysis using Imaris showed faster EB1 comets (0.13 μm/sec) after Neo1 knockdown as compared to control cells (0.10 μm/sec), p-value = 0.0001. (e) EB1 comets track length and duration was significantly longer in Neo1-siRNA treated cells, p-value < 0.0001.

Article Snippet: C-terminal GFP-tagged human EB1 plasmid (Cat No. 39299) was purchased from Addgene (Cambridge, MA).

Techniques: Knockdown, Binding Assay, Immunostaining, Western Blot, Control

Reagents used in the investigation.

Journal: Autophagy

Article Title: Regulation of N-degron recognin-mediated autophagy by the SARS-CoV-2 PLpro ubiquitin deconjugase

doi: 10.1080/15548627.2024.2442849

Figure Lengend Snippet: Reagents used in the investigation.

Article Snippet: pEGFP N1 Ub-CRT , Addgene , 69619; Marta Hallak lab.

Techniques: Recombinant, Diagnostic Assay, DC Protein Assay, Transfection, Mutagenesis, Plasmid Preparation, Gel Extraction, Purification, Cell Culture, shRNA